In Situ Identification of Unknown Crystals in Acute Kidney Injury Using Raman Spectroscopy.

ABSTRACT: Raman spectroscopy is a well-established and powerful tool for in situ biomolecular evaluation. Type 2 crystal nephropathies are characterized by the deposition of crystalline materials in the tubular lumen, resulting in rapid onset of acute kidney injury without specific symptoms. Timely crystal identification is essential for its diagnosis, mechanism exploration and therapy, but remains challenging. This study aims to develop a Raman spectroscopy-based method to assist pathological diagnosis of type 2 crystal nephropathies. Unknown crystals in renal tissue slides from a victim suffered extensive burn injury were detected by Raman spectroscopy, and the inclusion of crystals was determined by comparing Raman data with established database. Multiple crystals were scanned to verify the repro

SUBMITTER: Yu Y 

PROVIDER: S-EPMC9323692 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature