Tissue Barrier-on-Chip: A Technology for Reproducible Practice in Drug Testing.

ABSTRACT: One key application of organ-on-chip systems is the examination of drug transport and absorption through native cell barriers such the blood-brain barrier. To overcome previous hurdles related to the transferability of existing static cell cultivation protocols and polydimethylsiloxane (PDMS) as the construction material, a chip platform with key innovations for practical use in drug-permeation testing is presented. First, the design allows for the transfer of barrier-forming tissue into the microfluidic system after cells have been seeded on porous polymer or Si3N4 membranes. From this, we can follow highly reproducible models and cultivation protocols established for static drug testing, from coating the membrane to seeding the cells and cell analysis. Second, the perfusion system is a m

SUBMITTER: Koch EV 

PROVIDER: S-EPMC9323870 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature