Development and Evaluation of an Anti-Biotin Interference Method in Biotin-Streptavidin Immunoassays.

ABSTRACT: The strong non-covalent interaction between biotin and streptavidin places streptavidin-based assays, used by many laboratories, at an increased risk of interference by biotin. At present, a few manufacturers have developed fully automated anti-biotin interference methods, although compared with many detection platforms, these remain insufficient. Additionally, there is a need for more methods that can achieve fully automated anti-biotin interference. We sought to develop and evaluate a new biotin interference-resisting method based on a biotin-streptavidin chemiluminescence immunoassay. Streptavidin-coated magnetic microparticles (M) of different concentrations were prepared and tested for their biotin-resistance capabilities in an automated setting (Cobas e 601). The precision, accuracy,

SUBMITTER: Liu D 

PROVIDER: S-EPMC9324022 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature