Dataset Information

The Investigation of HBV Pre-S/S Gene Mutations in Occult HBV Infected Blood Donors with anti-HBs Positive.

ABSTRACT:

Introduction

The coexistence of hepatitis B virus (HBV) and hepatitis B surface antibodies (anti-HBs) in occult hepatitis B virus infection (OBI) is a contradictory phenomenon, and the underlying mechanism is not fully understood. The characteristics of pre-S/S mutations in OBI genotypes B and C (OBI_B and OBI_C) in the presence or absence of anti-HBs were analyzed extensively in this study. Methodology. The amino acid substitutions of envelope proteins of 21 OBI strains, including 4 HBs (+) OBI_B, 6 HBs (-) OBI_B, 6 HBs (+) OBIc, and 5 HBs (-) OBI_C samples, were analyzed and fully compared among groups of HBV genotypes and the presence of anti-HBs.

Results

The mutation rates in pre-S1, pre-S2, and S proteins of OBI_C were significantly higher than wild-type HBV (wt-HBV) genotype C strains, but only the mutation rate of S protein in OBI_B was significantly higher compared to wild-type HBV genotype B. The mutation rates in S protein of anti-HBs (-) OBI were higher than anti-HBs(+) OBI samples (4.40% vs. 2.43% in genotype B, P > 0.05; 6.81% vs. 3.47% in genotype C, P < 0.05). For these high-frequency substitutions in the pre-S/S region, the mutations sN40S and sK122R were found in 27.3% and 45.5% of anti-HBs (-) OBI strains, respectively. 7 substitutions were uniquely found in OBI_C strains and 9 substitutions were commonly detected in OBI_B and OBI_C strains.

Conclusions

These results suggested that the mutations might occur randomly and were not selected by antibody pressure.

SUBMITTER: Guo Y

PROVIDER: S-EPMC9325327 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The Investigation of HBV Pre-S/S Gene Mutations in Occult HBV Infected Blood Donors with anti-HBs Positive.

Guo Yan Y Lan Yu Y Jing Yuanyuan Y Cai Bin B Gong Hanshi H Zhang Yixin Y Duan Yong Y

The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale 20220719

<h4>Introduction</h4>The coexistence of hepatitis B virus (HBV) and hepatitis B surface antibodies (anti-HBs) in occult hepatitis B virus infection (OBI) is a contradictory phenomenon, and the underlying mechanism is not fully understood. The characteristics of pre-S/S mutations in OBI genotypes B and C (OBI<sub>B</sub> and OBI<sub>C</sub>) in the presence or absence of anti-HBs were analyzed extensively in this study. <i>Methodology</i>. The amino acid substitutions of envelope proteins of 21 O ...[more]

PMID: 35903154

Similar Datasets

Project description:BackgroundHepatocellular carcinoma (HCC) can develop in patients who are negative for the hepatitis B surface antigen (HBsAg) in serum but positive for hepatitis B virus (HBV) DNA in the liver, referred to as occult HBV infection (OBI). Previous reports showed that HBV variants in OBI-related HCC are different from those in HBsAg-positive HCC. In the present study, HBV quasispecies based on the pre-S/S gene in OBI-related HCC patients were examined by high throughput sequencing and compared with those in HBsAg-positive HCC.MethodsNineteen tissue samples (9 OBI-related and 10 HBsAg-positive non-cancerous tissues) were collected at the time of surgery at Kobe University Hospital. The quasispecies with more than 1% variation in the pre-S/S region were isolated and analysed by ultra-deep sequencing.ResultsThere were no significant differences in the major HBV populations, which exhibit more than 20% variation within the entire pre-S/S region, between OBI-related HCC and HBsAg-positive HCC. However, the prevalences of major populations with pre-S2 region mutations and of minor populations with polymerized human serum albumin-binding domain mutations were significantly higher in OBI-related HCC than in HBsAg-positive HCC. Moreover, the major variant populations associated with the B-cell epitope, located within the pre-S1 region, and the a determinant domain, located in the S region, were detected frequently in HBsAg-positive HCC. The minor populations of variants harbouring the W4R, L30S, Q118R/Stop, N123D and S124F/P mutations in the pre-S region and the L21F/S and L42F/S mutations in the S region were detected more frequently in OBI-related HCC than in HBsAg-positive HCC.ConclusionsUltra-deep sequencing revealed that the B-cell epitope domain in the pre-S1 region and alpha determinant domain in the S region were variable in HBsAg-positive HCC, although the quasispecies associated with the pre-S2 region were highly prevalent in OBI-related HCC.Trial registrationRef: R000034382/UMIN000030113; Retrospectively registered 25 November 2017.

Dataset Information

The Investigation of HBV Pre-S/S Gene Mutations in Occult HBV Infected Blood Donors with anti-HBs Positive.

Introduction

Results

Conclusions

Publications

The Investigation of HBV Pre-S/S Gene Mutations in Occult HBV Infected Blood Donors with anti-HBs Positive.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets