Dataset Information

Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola.

ABSTRACT: Canola varieties exhibit variation in drought avoidance and drought escape traits, reflecting adaptation to water-deficit environments. Our understanding of underlying genes and their interaction across environments in improving crop productivity is limited. A doubled haploid population was analysed to identify quantitative trait loci (QTL) associated with water-use efficiency (WUE) related traits. High WUE in the vegetative phase was associated with low seed yield. Based on the resequenced parental genome data, we developed sequence-capture-based markers and validated their linkage with carbon isotope discrimination (Δ¹³ C) in an F₂ population. RNA sequencing was performed to determine the expression of candidate genes underlying Δ¹³ C QTL. QTL contributing to main and QTL × environment interaction effects for Δ¹³ C and yield were identified. One multiple-trait QTL for Δ¹³ C, days to flower, plant height, and seed yield was identified on chromosome A09. Interestingly, this QTL region overlapped with a homoeologous exchange (HE) event, suggesting its association with the multiple traits. Transcriptome analysis revealed 121 significantly differentially expressed genes underlying Δ¹³ C QTL on A09 and C09, including in HE regions. Sorting out the negative relationship between vegetative WUE and seed yield is a priority. Genetic and genomic resources and knowledge so developed could improve canola WUE and yield.

SUBMITTER: Raman H

PROVIDER: S-EPMC9325393 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola.

Raman Harsh H Raman Rosy R Pirathiban Ramethaa R McVittie Brett B Sharma Niharika N Liu Shengyi S Qiu Yu Y Zhu Anyu A Kilian Andrzej A Cullis Brian B Farquhar Graham D GD Stuart-Williams Hilary H White Rosemary R Tabah David D Easton Andrew A Zhang Yuanyuan Y

Plant, cell & environment 20220505 7

Canola varieties exhibit variation in drought avoidance and drought escape traits, reflecting adaptation to water-deficit environments. Our understanding of underlying genes and their interaction across environments in improving crop productivity is limited. A doubled haploid population was analysed to identify quantitative trait loci (QTL) associated with water-use efficiency (WUE) related traits. High WUE in the vegetative phase was associated with low seed yield. Based on the resequenced pare ...[more]

PMID: 35445756

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Project description:Photosynthetic assimilation is remarkably altered by heat and drought, and this depends on the individual or combined occurrence of stressors and their respective intensities and durations. Abiotic stressors may also alter the nutritional quality and economic value of crops. In this controlled greenhouse study, we evaluated the response of Brassica napus L., from flowering to seed development, to two temperature and water treatments and a combination of these treatments. The diffusional limitations of stomatal conductance and mesophyll conductance on photosynthesis, as well as resource-use efficiency (particularly water and nitrogen), were assessed. In addition, the effects of stressors on the seed fatty acid content and composition and the total protein content were examined. The results showed that the reduction in the net photosynthetic assimilation rate was caused by combinations of heat and drought (heat + drought) treatments, by drought alone, and, to a lesser extent, by heat alone. The stomatal conductance decreased under drought and heat + drought treatments but not under heat. Conversely, the mesophyll conductance was reduced significantly in the plants exposed to heat and heat + drought but not in the plants exposed to drought alone. The carboxylation efficiency rate and the electron transport rate were reduced under the heat treatment. The seed yield was reduced by 85.3% under the heat treatment and, to a lesser extent, under the drought treatment (31%). This emphasizes the devastating effects of hotter weather on seed formation and development. Seed oil content decreased by 52% in the plants exposed to heat, the protein content increased under all the stress treatments. Heat treatment had a more deleterious effect than drought on the seed oil composition, leading to enhanced levels of saturated fatty oils and, consequently, desaturation efficiency, a measure of oil frying ability. Overall, this study showed that except for the photosynthetic assimilation rate and stomatal conductance, heat, rather than drought, negatively affected the photosynthetic capacity, yield, and oil quality attributes when imposed during the flowering and silique-filling stages. This result highlights the necessity for a better understanding of heat tolerance mechanisms in crops to help to create germplasms that are adapted to rapid climate warming.

Dataset Information

Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola.

Publications

Multienvironment QTL analysis delineates a major locus associated with homoeologous exchanges for water-use efficiency and seed yield in canola.

Similar Datasets

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