Non-canonical endogenous expression of voltage-gated sodium channel Na_V 1.7 subtype by the TE671 rhabdomyosarcoma cell line.

ABSTRACT: The human TE671 cell line was originally used as a model of medulloblastoma but has since been reassigned as rhabdomyosarcoma. Despite the characterised endogenous expression of voltage-sensitive sodium currents in these cells, the specific voltage-gated sodium channel (VGSC) subtype underlying these currents remains unknown. To profile the VGSC subtype in undifferentiated TE671 cells, endpoint and quantitative reverse transcription-PCR (qRT-PCR), western blot and whole-cell patch clamp electrophysiology were performed. qRT-PCR profiling revealed that expression of the SCN9A gene was ∼215-fold greater than the SCN4A gene and over 400-fold greater than any of the other VGSC genes, while western blot confirmed that the dominant SCN9A RNA was translated to a protein with a molecular mass of ∼

SUBMITTER: Ngum NM 

PROVIDER: S-EPMC9325523 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature