Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells.

ABSTRACT: The retinal pigment epithelium (RPE) is a polarized monolayer that secretes growth factors and cytokines towards the retina apically and the choroid basolaterally. Numerous RPE secreted proteins have been linked to the pathogenesis of age-related macular degeneration (AMD). The purpose of this study was to determine the differential apical and basolateral secretome of RPE cells, and the effects of oxidative stress on directional secretion of proteins linked to AMD and angiogenesis. Tandem mass tag spectrometry was used to profile proteins in human iPSC-RPE apical and basolateral conditioned media. Changes in secretion after oxidative stress induced by H₂O₂ or tert-butyl hydroperoxide (tBH) were investigated by ELISA and western analysis. Out of 926 differentially secr

SUBMITTER: Chen L 

PROVIDER: S-EPMC9325713 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature