Theileria annulata histone deacetylase 1 (TaHDAC1) initiates schizont to merozoite stage conversion.

ABSTRACT: A fungal metabolite, FR235222, specifically inhibits a histone deacetylase of the apicomplexan parasite Toxoplasma gondii and TgHDAC3 has emerged as a key factor regulating developmental stage transition in this species. Here, we exploited FR235222 to ask if changes in histone acetylation regulate developmental stage transition of Theileria annulata, another apicomplexan species. We found that FR235222 treatment of T. annulata-infected transformed leukocytes induced a proliferation arrest. The blockade in proliferation was due to drug-induced conversion of intracellular schizonts to merozoites that lack the ability to maintain host leukocyte cell division. Induction of merogony by FR235222 leads to an increase in expression of merozoite-marker (rhoptry) proteins. RNA-seq of FR235222-treate

SUBMITTER: Tajeri S 

PROVIDER: S-EPMC9325746 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature