Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.

ABSTRACT:

Introduction

Colorectal cancer remains a public health issue and most colon cancer patients succumb to the development of metastases. Using a specific protocol of pressure-assisted interstitial fluid extrusion to recover soluble biomarkers, we identified paladin as a potential colon cancer liver metastases biomarker.

Methods

Using shRNA gene knockdown, we explored the biological function of paladin in colon cancer cells and investigated the phospho-proteome within colon cancer cells. We successively applied in vitro migration assays, in vivo metastasis models and co-immunoprecipitation experiments.

Results

We discovered that paladin is required for colon cancer cell migration and metastasis, and that paladin depletion altered the phospho-proteome within colon cancer c

SUBMITTER: Rademaker G 

PROVIDER: S-EPMC9325978 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature