Transcriptomic Complexity in Strawberry Fruit Development and Maturation Revealed by Nanopore Sequencing.

ABSTRACT: The use of alternative transcription start or termination sites (aTSS or aTTS) as well as alternative splicing (AS) produce diverse transcript isoforms, playing indispensable roles in the plant development and environmental adaptations. Despite the advances in the finding of the genome-wide alternatively spliced genes in strawberry, it remains unexplored how AS responds to the developmental cues and what relevance do these outcomes have to the gene function. In this study, we have systematically investigated the transcriptome complexity using long-read Oxford Nanopore Technologies along the four successive developmental stages. The full-length cDNA sequencing results unraveled thousands of previously unexplored transcript isoforms raised from aTSS, aTTS, and AS. The relative contributions

SUBMITTER: Chen Q 

PROVIDER: S-EPMC9326444 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature