Transforming Chemical Proteomics Enrichment into a High-Throughput Method Using an SP2E Workflow.

ABSTRACT: Protein post-translational modifications (PTMs) play a critical role in the regulation of protein catalytic activity, localization, and protein-protein interactions. Attachment of PTMs onto proteins significantly diversifies their structure and function, resulting in proteoforms. However, the sole identification of post-translationally modified proteins, which are often cell type and disease-specific, is still a highly challenging task. Substoichiometric amounts and modifications of low abundant proteins necessitate the purification or enrichment of the modified proteins. Although the introduction of mass spectrometry-based chemical proteomic strategies has enabled the screening of protein PTMs with increased throughput, sample preparation remains highly time-consuming and tedious. Here, w

SUBMITTER: Becker T 

PROVIDER: S-EPMC9326820 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature