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Achieving Selectivity for Phosphate over Pyrophosphate in Ethanol with Iron(III)-Based Fluorescent Probes.

ABSTRACT: Two iron(III)-based molecular receptors employing 1,2-hydroxypyridinone ligands were developed for phosphate recognition and fluorescence sensing via indicator displacement assay (IDA). The tetra- and pentadentate ligands enable anion recognition by the iron(III) center via its remaining one or two open coordination sites. Weak protective coordination of fluorescein at those sites prevents the formation of μ-oxo dimers in aerated solutions. Its rapid and selective displacement by inorganic phosphate results in a 20-fold increase in the fluorescence of the indicator. Both receptors exhibit high affinity for inorganic phosphate and high selectivity over common competing anions, including halides, acetate, carbonate, and, remarkably, pyrophosphate as well as arsenate. Coordination of phosphate to the iron(III) center was confirmed by ATR-IR and ³¹P NMR spectroscopy.

SUBMITTER: Huang SY

PROVIDER: S-EPMC9326827 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Achieving Selectivity for Phosphate over Pyrophosphate in Ethanol with Iron(III)-Based Fluorescent Probes.

Huang Sheng-Yin SY Pierre Valérie C VC

JACS Au 20220625 7

Two iron(III)-based molecular receptors employing 1,2-hydroxypyridinone ligands were developed for phosphate recognition and fluorescence sensing via indicator displacement assay (IDA). The tetra- and pentadentate ligands enable anion recognition by the iron(III) center via its remaining one or two open coordination sites. Weak protective coordination of fluorescein at those sites prevents the formation of μ-oxo dimers in aerated solutions. Its rapid and selective displacement by inorganic phosp ...[more]

PMID: 35911450

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