Regulation of eotaxin expression in skin allergic diseases.

ABSTRACT:

Introduction

As a key chemotactic factor during Eos recruitment on the allergic inflammation site, eotaxin is regarded as one of the important therapeutic targets.

Aim

To address the expression and regulation mechanism of eotaxin, which constitutes an important procedure in skin allergic disease and a target for drug therapy.

Material and methods

An allergic contact dermatitis (ACD) model of mouse was established. Immunohistochemical method (ICH) and flow cytometry method (FCM) were used to determine the amounts of CD4+ and CD8+ T cells and their ratios. The eotaxin mRNA and protein were evaluated by real-time PCR, ICH and western-blotting method. Nuclear factor-κB (NF-κB) nuclear translocation and STAT6 phosphorylation were studied by EMSA and western-blotting method

SUBMITTER: Peng C 

PROVIDER: S-EPMC9326924 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature