Transplantation of insulin-producing cells derived from human mesenchymal stromal/stem cells into diabetic humanized mice.

ABSTRACT:

Background

The purpose of this study was to investigate allogenic immune responses following the transplantation of insulin-producing cells (IPCs) differentiated from human adipose tissue-derived stem cells (hAT-MSCs) into humanized mice.

Methods

hAT-MSCs were isolated from liposuction aspirates obtained from HLA-A2-negative healthy donors. These cells were expanded and differentiated into IPCs. HLA-A2-positive humanized mice (NOG-EXL) were divided into 4 groups: diabetic mice transplanted with IPCs, diabetic but nontransplanted mice, nondiabetic mice transplanted with IPCs and normal untreated mice. Three million differentiated cells were transplanted under the renal capsule. Animals were followed-up to determine their weight, glucose levels (2-h postprandial), and human an

SUBMITTER: Ghoneim MA 

PROVIDER: S-EPMC9327173 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature