Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of H₂O₂ enhances their ability to treat intervertebral disc degeneration.

ABSTRACT:

Background

Nucleus pulposus mesenchymal stem cells (NPMSCs) transplantation is a promising treatment for intervertebral disc degeneration (IVDD). However, the transplanted NPMSCs exhibited weak cell proliferation, high cell apoptosis, and a low ability to resist the harsh microenvironment of the degenerated intervertebral disc. There is an urgent need to explore feasible methods to enhance the therapeutic efficacy of NPMSCs transplantation.

Objective

To identify the optimal concentration for NPMSCs pretreatment with hydrogen peroxide (H₂O₂) and explore the therapeutic efficacy of NPMSCs transplantation using H₂O₂ pretreatment in IVDD.

Methods

Rat NPMSCs were pretreated with different concentrations (range from 25 to 300 μM)

SUBMITTER: Zhang YY 

PROVIDER: S-EPMC9327256 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature