Adipose-derived stromal cells reverse insulin resistance through inhibition of M1 expression in a type 2 diabetes mellitus mouse model.

ABSTRACT:

Background

Adipose tissue inflammation is considered as one of the major mechanisms underlying the pathogenesis of insulin resistance and complications in diabetes. Here, we aimed to study the effects of adipose-derived stromal cells on diabetes-induced insulin resistance and M1 cytokine expression.

Methods

Stromal vascular fractions (SVFs) purified from the inguinal adipose tissue of diabetic mice were treated with plasma from either nondiabetic (Lepr^+/+) or diabetic (Lepr^db/db) mice and injected into the inguinal white adipose tissue of Lepr^db/db mice.

Results

We found that diabetic plasma treatment induced, whereas nondiabetic plasma suppressed TNF-α, IL-1β, and dipeptidyl peptidase 4 (DPP4) mRNA expression in SVFs in vitro. Importa

SUBMITTER: Chen LW 

PROVIDER: S-EPMC9327360 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature