Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing.

ABSTRACT:

Background

The single-cell platform provided revolutionary way to study cellular biology. Technologically, a sophistic protocol of isolating qualified single cells would be key to deliver to single-cell platform, which requires high cell viability, high cell yield and low content of cell aggregates or doublets. For musculoskeletal tissues, like bone, cartilage, nucleus pulposus and tendons, as well as their pathological state, which are tense and dense, it's full of challenge to efficiently and rapidly prepare qualified single-cell suspension. Conventionally, enzymatic dissociation methods were wildly used but lack of quality control. In the present study, we designed the rapid cycling enzymatic processing method using tissue-specific enzyme cocktail to treat different human pathol

SUBMITTER: Gao M 

PROVIDER: S-EPMC9327421 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature