The Capsid Precursor Protein of Astrovirus VA1 Is Proteolytically Processed Intracellularly.

ABSTRACT: Human astrovirus VA1 has been associated with neurological disease in immunocompromised patients, and its recent propagation in cell culture has opened the possibility to study its biology. Unlike classical human astroviruses, VA1 growth was found to be independent of trypsin during virus replication in vitro. In this work, we show that despite its independence on trypsin activation for cell infection, the VA1 capsid precursor protein, of 86 kDa (VP86), is processed intracellularly, and this proteolytic processing is important for astrovirus VA1 infectivity. Antibodies raised against different regions of the capsid precursor showed that the polyprotein can be processed starting at either its amino- or carboxy-terminal end, and they allowed us to identify those proteins of about 33 (

SUBMITTER: Aguilera-Flores C 

PROVIDER: S-EPMC9327696 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature