Dataset Information

Complete mitochondrial DNA sequence of Golden trevally Gnathanodon speciosus (Forsskal, 1775) and the phylogenetic analysis of Carangidae.

ABSTRACT: In this study, the complete mitochondrial genome of Gnathanodon speciosus was determined. The entire mitochondrial DNA sequence is 16,555 bp in length and consists of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region. Phylogenetic analysis was performed using 13 PCGs showing that G. speciosus belongs to the Carangidae family, and is most closely related to the species in genera Caranx and Megalaspis.

SUBMITTER: Jiang F

PROVIDER: S-EPMC9327777 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Complete mitochondrial DNA sequence of Golden trevally Gnathanodon speciosus (Forsskål, 1775) and the phylogenetic analysis of Carangidae.

Jiang Fangyan F Yang Ning N Huang Hai H

Mitochondrial DNA. Part B, Resources 20220725 7

In this study, the complete mitochondrial genome of Gnathanodon speciosus was determined. The entire mitochondrial DNA sequence is 16,555 bp in length and consists of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region. Phylogenetic analysis was performed using 13 PCGs showing that G. speciosus belongs to the Carangidae family, and is most closely related to the species in genera Caranx and Megalaspis. ...[more]

PMID: 35911468

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Project description:BackgroundThe genetic control of sex determination in teleost species is poorly understood. This is partly because of the diversity of mechanisms that determine sex in this large group of vertebrates, including constitutive genes linked to sex chromosomes, polygenic constitutive mechanisms, environmental factors, hermaphroditism, and unisexuality. Here we use a de novo genome assembly of New Zealand silver trevally (Pseudocaranx georgianus) together with sex-specific whole genome sequencing data to detect sexually divergent genomic regions, identify candidate genes and develop molecular makers.ResultsThe de novo assembly of an unsexed trevally (Trevally_v1) resulted in a final assembly of 579.4 Mb in length, with a N50 of 25.2 Mb. Of the assembled scaffolds, 24 were of chromosome scale, ranging from 11 to 31 Mb in length. A total of 28,416 genes were annotated after 12.8 % of the assembly was masked with repetitive elements. Whole genome re-sequencing of 13 wild sexed trevally (seven males and six females) identified two sexually divergent regions located on two scaffolds, including a 6 kb region at the proximal end of chromosome 21. Blast analyses revealed similarity between one region and the aromatase genes cyp19 (a1a/b) (E-value < 1.00E-25, identity > 78.8 %). Males contained higher numbers of heterozygous variants in both regions, while females showed regions of very low read-depth, indicative of male-specificity of this genomic region. Molecular markers were developed and subsequently tested on 96 histologically-sexed fish (42 males and 54 females). Three markers amplified in absolute correspondence with sex (positive in males, negative in females).ConclusionsThe higher number of heterozygous variants in males combined with the absence of these regions in females support a XY sex-determination model, indicating that the trevally_v1 genome assembly was developed from a male specimen. This sex system contrasts with the ZW sex-determination model documented in closely related carangid species. Our results indicate a sex-determining function of a cyp19a1a-like gene, suggesting the molecular pathway of sex determination is somewhat conserved in this family. The genomic resources developed here will facilitate future comparative work, and enable improved insights into the varied sex determination pathways in teleosts. The sex marker developed in this study will be a valuable resource for aquaculture selective breeding programmes, and for determining sex ratios in wild populations.

Dataset Information

Complete mitochondrial DNA sequence of Golden trevally Gnathanodon speciosus (Forsskal, 1775) and the phylogenetic analysis of Carangidae.

Publications

Complete mitochondrial DNA sequence of Golden trevally <i>Gnathanodon speciosus</i> (Forsskål, 1775) and the phylogenetic analysis of Carangidae.

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