Inducible and tissue-specific cell labeling in Cre-ER^T2 transgenic Xenopus lines.

ABSTRACT: Investigating cell lineage requires genetic tools that label cells in a temporal and tissue-specific manner. The bacteriophage-derived Cre-ER^T2 /loxP system has been developed as a genetic tool for lineage tracing in many organisms. We recently reported a stable transgenic Xenopus line with a Cre-ER^T2 /loxP system driven by the mouse Prrx1 (mPrrx1) enhancer to trace limb fibroblasts during the regeneration process (Prrx1:CreER line). Here we describe the detailed technological development and characterization of such line. Transgenic lines carrying a CAG promoter-driven Cre-ER^T2 /loxP system showed conditional labeling of muscle, epidermal, and interstitial cells in both the tadpole tail and the froglet leg upon 4-hydroxytamoxifen (4OHT) treatment. We furth

SUBMITTER: Lin TY 

PROVIDER: S-EPMC9328194 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature