Carbenoxolone and 18β-glycyrrhetinic acid inhibit inositol 1,4,5-trisphosphate-mediated endothelial cell calcium signalling and depolarise mitochondria.

ABSTRACT:

Background and purpose

Coordinated endothelial control of cardiovascular function is proposed to occur by endothelial cell communication via gap junctions and connexins. To study intercellular communication, the pharmacological agents carbenoxolone (CBX) and 18β-glycyrrhetinic acid (18βGA) are used widely as connexin inhibitors and gap junction blockers.

Experimental approach

We investigated the effects of CBX and 18βGA on intercellular Ca²⁺ waves, evoked by inositol 1,4,5-trisphosphate (IP₃ ) in the endothelium of intact mesenteric resistance arteries.

Key results

Acetycholine-evoked IP₃ -mediated Ca²⁺ release and propagated waves were inhibited by CBX (100 μM) and 18βGA (40 μM). Unexpectedly, the Ca²⁺ signals we

SUBMITTER: Buckley C 

PROVIDER: S-EPMC9328419 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature