A non-transcriptional function of Yap regulates the DNA replication program in Xenopus laevis.

ABSTRACT: In multicellular eukaryotic organisms, the initiation of DNA replication occurs asynchronously throughout S-phase according to a regulated replication timing program. Here, using Xenopus egg extracts, we showed that Yap (Yes-associated protein 1), a downstream effector of the Hippo signalling pathway, is required for the control of DNA replication dynamics. We found that Yap is recruited to chromatin at the start of DNA replication and identified Rif1, a major regulator of the DNA replication timing program, as a novel Yap binding protein. Furthermore, we show that either Yap or Rif1 depletion accelerates DNA replication dynamics by increasing the number of activated replication origins. In Xenopus embryos, using a Trim-Away approach during cleavage stages devoid of transcrip

SUBMITTER: Melendez Garcia R 

PROVIDER: S-EPMC9328763 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature