Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex I.

ABSTRACT: Oocytes form before birth and remain viable for several decades before fertilization¹. Although poor oocyte quality accounts for most female fertility problems, little is known about how oocytes maintain cellular fitness, or why their quality eventually declines with age². Reactive oxygen species (ROS) produced as by-products of mitochondrial activity are associated with lower rates of fertilization and embryo survival^3-5. Yet, how healthy oocytes balance essential mitochondrial activity with the production of ROS is unknown. Here we show that oocytes evade ROS by remodelling the mitochondrial electron transport chain through elimination of complex I. Combining live-cell imaging and proteomics in human and Xenopus oocytes, we find that early oocytes exhibit

SUBMITTER: Rodriguez-Nuevo A 

PROVIDER: S-EPMC9329100 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature