Toll-like Receptor 9 Pathway Mediates Schlafen⁺-MDSC Polarization During Helicobacter-induced Gastric Metaplasias.

ABSTRACT:

Background & aims

A subset of myeloid-derived suppressor cells (MDSCs) that express murine Schlafen4 (SLFN4) or its human ortholog SLFN12L polarize in the Helicobacter-inflamed stomach coincident with intestinal or spasmolytic polypeptide-expressing metaplasia. We propose that individuals with a more robust response to damage-activated molecular patterns and increased Toll-like receptor 9 (TLR9) expression are predisposed to the neoplastic complications of Helicobacter infection.

Methods

A mouse or human Transwell co-culture system composed of dendritic cells (DCs), 2-dimensional gastric epithelial monolayers, and Helicobacter were used to dissect the cellular source of interferon-α (IFNα) in the stomach by flow cytometry. Conditioned media from the co-cultures polarized pri

SUBMITTER: Ding L 

PROVIDER: S-EPMC9329252 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature