Metabolomics analysis of milk thistle lipids to identify drought-tolerant genes.
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ABSTRACT: Milk thistle is an oil and medicinal crop known as an alternative oil crop with a high level of unsaturated fatty acids, which makes it a favorable edible oil for use in food production. To evaluate the importance of Milk thistle lipids in drought tolerance, an experiment was performed in field conditions under three different water deficit levels (Field capacity (FC), 70% FC and 40% FC). After harvesting seeds of the plant, their oily and methanolic extracts were isolated, and subsequently, types and amounts of lipids were measured using GC-MS. Genes and enzymes engaged in biosynthesizing of these lipids were identified and their expression in Arabidopsis was investigated under similar conditions. The results showed that content of almost all measured lipids of milk thistle decreased unde
SUBMITTER: Ghanbari Moheb Seraj R
PROVIDER: S-EPMC9329356 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
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