A host-vector toolbox for improved secretory protein overproduction in Bacillus subtilis.

ABSTRACT: Target proteins in biotechnological applications are highly diverse. Therefore, versatile flexible expression systems for their functional overproduction are required. In order to find the right heterologous gene expression strategy, suitable host-vector systems, which combine different genetic circuits, are useful. In this study, we designed a novel Bacillus subtilis expression toolbox, which allows the overproduction and secretion of potentially toxic enzymes. This toolbox comprises a set of 60 expression vectors, which combine two promoter variants, four strong secretion signals, a translation-enhancing downstream box, and three plasmid backbones. This B. subtilis toolbox is based on a tailor-made, clean deletion mutant strain, which is protease and sporulation deficient and exhibits re

SUBMITTER: Kruger A 

PROVIDER: S-EPMC9329435 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature