NBBt-test: a versatile method for differential analysis of multiple types of RNA-seq data.

ABSTRACT: Rapid development of transcriptome sequencing technologies has resulted in a data revolution and emergence of new approaches to study transcriptomic regulation such as alternative splicing, alternative polyadenylation, CRISPR knockout screening in addition to the regular gene expression. A full characterization of the transcriptional landscape of different groups of cells or tissues holds enormous potential for both basic science as well as clinical applications. Although many methods have been developed in the realm of differential gene expression analysis, they all geared towards a particular type of sequencing data and failed to perform well when applied in different types of transcriptomic data. To fill this gap, we offer a negative beta binomial t-test (NBBt-test). NBBt-test provides

SUBMITTER: Tan YD 

PROVIDER: S-EPMC9329447 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature