Real-time precision opto-control of chemical processes in live cells.

ABSTRACT: Precision control of molecular activities and chemical reactions in live cells is a long-sought capability by life scientists. No existing technology can probe molecular targets in cells and simultaneously control the activities of only these targets at high spatial precision. We develop a real-time precision opto-control (RPOC) technology that detects a chemical-specific optical response from molecular targets during laser scanning and uses the optical signal to couple a separate laser to only interact with these molecules without affecting other sample locations. We demonstrate precision control of molecular states of a photochromic molecule in different regions of the cells. We also synthesize a photoswitchable compound and use it with RPOC to achieve site-specific inhibition of microtu

SUBMITTER: Clark MG 

PROVIDER: S-EPMC9329476 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature