Template-Based Assembly of Proteomic Short Reads For De Novo Antibody Sequencing and Repertoire Profiling.

ABSTRACT: Antibodies can target a vast molecular diversity of antigens. This is achieved by generating a complementary diversity of antibody sequences through somatic recombination and hypermutation. A full understanding of the antibody repertoire in health and disease therefore requires dedicated de novo sequencing methods. Next-generation cDNA sequencing methods have laid the foundation of our current understanding of the antibody repertoire, but these methods share one major limitation in that they target the antibody-producing B-cells, rather than the functional secreted product in bodily fluids. Mass spectrometry-based methods offer an opportunity to bridge this gap between antibody repertoire profiling and bulk serological assays, as they can access antibody sequence information straigh

SUBMITTER: Schulte D 

PROVIDER: S-EPMC9330293 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature