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A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury.


ABSTRACT: We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP's protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe2+ level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.

SUBMITTER: He P 

PROVIDER: S-EPMC9330552 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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A Novel Polysaccharide From <i>Chuanminshen violaceum</i> and Its Protective Effect Against Myocardial Injury.

He Peng P   Zhang Mi M   Zhao Meng M   Zhang Mengyao M   Ma Benxu B   Lv Hongyu H   Han Yantao Y   Wu Dingtao D   Zhong Zhangfeng Z   Zhao Wenwen W  

Frontiers in nutrition 20220714


We isolated and purified a novel polysaccharide from the root of <i>Chuanminshen violaceum</i>, namely, <i>Chuanminshen violaceumis</i> polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, <i>in vivo</i> experiment, CVP's protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function  ...[more]

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