Gene-Regulatory Potential of 25-Hydroxyvitamin D₃ and D₂.

ABSTRACT: Human peripheral blood mononuclear cells (PBMCs) represent a highly responsive primary tissue that is composed of innate and adaptive immune cells. In this study, we compared modulation of the transcriptome of PBMCs by the vitamin D metabolites 25-hydroxyvitamin D₃ (25(OH)D₃), 25(OH)D₂ and 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃). Saturating concentrations of 1,25(OH)₂D₃, 25(OH)D₃ and 25(OH)D₂ resulted after 24 h stimulation in a comparable number and identity of target genes, but below 250 nM 25(OH)D₃ and 25(OH)D₂ were largely insufficient to affect the transcriptome. The average EC₅₀ values of 206 common target genes were 322 nM for 25(OH)D_3</

SUBMITTER: Hanel A 

PROVIDER: S-EPMC9330572 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature