Lack of Nck1 protein and Nck-CD3 interaction caused the increment of lipid content in Jurkat T cells.

ABSTRACT:

Background

The non-catalytic region of tyrosine kinase (Nck) is an adaptor protein, which is ubiquitously expressed in many types of cells. In T cells, the Nck1 isoform promotes T cell receptor signalling as well as actin polymerisation. However, the role of Nck1 in the lipid metabolism in T cells is unknown. In the present study, we investigated the effect of the Nck1 protein and Nck-CD3 interaction on lipid metabolism and on the physical and biological properties of Jurkat T cells, using a newly developed holotomographic microscope.

Results

Holotomographic microscopy showed that Nck1-knocked-out cells had membrane blebs and were irregular in shape compared to the rounded control cells. The cell size and volume of Nck1-deficient cells were comparable to those of the control

SUBMITTER: Nuiyen A 

PROVIDER: S-EPMC9330638 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature