Retinoic acid and FGF10 promote the differentiation of pluripotent stem cells into salivary gland placodes.

ABSTRACT:

Background

Salivary glands produce saliva that play essential roles in digestion and oral health. Derivation of salivary gland organoids from pluripotent stem cells (PSCs) provides a powerful platform to model the organogenesis processes during development. A few studies attempted to differentiate PSCs into salivary gland organoids. However, none of them could recapitulate the morphogenesis of the embryonic salivary glands, and most of the protocols involved complicated manufacturing processes.

Methods

To generate PSC-derived salivary gland placodes, the mouse embryonic stem cells were first differentiated into oral ectoderm by treatment with BMP4 on day 3. Retinoic acid and bFGF were then applied to the cultures from day 4 to day 6, followed by a 4-day treatment of FGF10. T

SUBMITTER: Zhang S 

PROVIDER: S-EPMC9330698 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature