Engineered Production of Isobutanol from Sugarcane Trash Hydrolysates in Pichia pastoris.

ABSTRACT: Concerns over climate change have led to increased interest in renewable fuels in recent years. Microbial production of advanced fuels from renewable and readily available carbon sources has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we engineered the yeast Pichia pastoris, an industrial powerhouse in heterologous enzyme production, to produce the advanced biofuel isobutanol from sugarcane trash hydrolysates. Our strategy involved overexpressing a heterologous xylose isomerase and the endogenous xylulokinase to enable the yeast to consume both C5 and C6 sugars in biomass. To enable the yeast to produce isobutanol, we then overexpressed the endogenous amino acid biosynthetic pathway and the 2-keto acid degradation pathway. The en

SUBMITTER: Bumrungtham P 

PROVIDER: S-EPMC9330720 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature