Expression and Prognostic Value of Melanoma-Associated Antigen D2 in Gliomas.

ABSTRACT:

Introduction

The melanoma-associated antigen D2 (MAGED2) is one of the melanoma-associated antigen family members. It is commonly overexpressed in a variety of malignancies. However, the mechanism and function of MAGED2 in glioma remain unknown.

Methods

The MAGED2 expression level and the correlations between clinical characteristics were analyzed with the data from the CGGA and TCGA datasets. MAGED2 expression in 98 glioma tissues was measured using RT-qPCR, Western blot, and immunohistochemistry. CCK-8, colony formation, and EdU assays were used to assess the effect of MAGED2 on U251-MG cell proliferation. Flow cytometry was used to track changes in the cell cycle and cell apoptosis following plasmid transfection with CRISPRi.

Results

MAGED2 was shown to be highly e

SUBMITTER: Yan J 

PROVIDER: S-EPMC9330880 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature