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Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8.

ABSTRACT: Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

SUBMITTER: Pettinari MJ 

PROVIDER: S-EPMC93310 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature

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Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8.

Pettinari M J MJ   Vázquez G J GJ   Silberschmidt D D   Rehm B B   Steinbüchel A A   Méndez B S BS  

Applied and environmental microbiology 20011101 11

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucos  ...[more]

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