Transcriptional and Epigenetic Consequences of DMSO Treatment on HepaRG Cells.

ABSTRACT: Dimethyl sulfoxide (DMSO) is used to sustain or favor hepatocyte differentiation in vitro. Thus, DMSO is used in the differentiation protocol of the HepaRG cells that present the closest drug-metabolizing enzyme activities to primary human hepatocytes in culture. The aim of our study is to clarify its influence on liver-specific gene expression. For that purpose, we performed a large-scale analysis (gene expression and histone modification) to determine the global role of DMSO exposure during the differentiation process of the HepaRG cells. The addition of DMSO drives the upregulation of genes mainly regulated by PXR and PPARα whereas genes not affected by this addition are regulated by HNF1α, HNF4α, and PPARα. DMSO-differentiated-HepaRG cells show a differential expression for genes regul

SUBMITTER: Dubois-Pot-Schneider H 

PROVIDER: S-EPMC9331440 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature