Reprogramming adipose mesenchymal stem cells into islet β-cells for the treatment of canine diabetes mellitus.

ABSTRACT:

Background

Islet transplantation is an excellent method for the treatment of type I diabetes mellitus. However, due to the limited number of donors, cumbersome isolation and purification procedures, and immune rejection, the clinical application is greatly limited. The development of a simple and efficient new method to obtain islet β-cells is a key problem that urgently requires a solution for the treatment of type I diabetes mellitus.

Methods

In this study, Pbx1, Rfx3, Pdx1, Ngn3, Pax4 and MafA were used to form a six-gene combination to efficiently reprogram aMSCs (adipose mesenchymal stem cells) into ra-βCs (reprogrammed aMSCs-derived islet β-cells), and the characteristics and immunogenicity of ra-βCs were detected. Feasibility of ra-βCs transplantation for the treatmen

SUBMITTER: Dai P 

PROVIDER: S-EPMC9331803 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature