Alternative Splicing Isoforms of Porcine CREB Are Differentially Involved in Transcriptional Transactivation.

ABSTRACT: The cAMP response element-binding protein (CREB), a basic leucine zipper transcription factor, is involved in the activation of numerous genes in a variety of cell types. The CREB gene is rich in alternative splicing (AS) events. However, studies on the AS of CREB genes in pigs are limited, and few reports have compared the roles of isoforms in activating gene expression. Here, five AS transcripts, V1-5, were characterized by RT-PCR and two, V3 and V5, were new identifications. Both V1 and V2 have all the functional domains of the CREB protein, with similar tissue expression profiles and mRNA stability, suggesting that they have similar roles. The transcriptional transactivation activities of four isoforms encoding complete polypeptides were analyzed on the expression of the

SUBMITTER: Zhang D 

PROVIDER: S-EPMC9331954 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature