Cre-Recombinase Induces Apoptosis and Cell Death in Enterocyte Organoids.

ABSTRACT: The culture of primary intestinal epithelia cells is not possible in a normal culture system. In 2009 a three-dimensional culture system of intestinal stem cells was established that shows many of the physiological features of the small intestine, such as crypt-villus structure, stem cell niche and all types of differentiated intestinal epithelial cells. These enteroids can be used to analyze biology of intestinal stem cells, gut homeostasis and the development of diseases. They also give the possibility to reduce animal numbers, as enteroids can be cryo-conserved and cultivated for many passages. To investigate the influence of genes such as NADPH oxidases on the gut homeostasis, transgenic approached are the method of choice. The generation of enteroids from knockout mice allows real-tim

SUBMITTER: Moll F 

PROVIDER: S-EPMC9332190 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature