Structure-Function Relationships in Temperature Effects on Bacterial Luciferases: Nothing Is Perfect.

ABSTRACT: The evaluation of temperature effects on the structure and function of enzymes is necessary to understand the mechanisms underlying their adaptation to a constantly changing environment. In the current study, we investigated the influence of temperature variation on the activity, structural dynamics, thermal inactivation and denaturation of Photobacterium leiognathi and Vibrio harveyi luciferases belonging to different subfamilies, as well as the role of sucrose in maintaining the enzymes functioning and stability. We used the stopped-flow technique, differential scanning calorimetry and molecular dynamics to study the activity, inactivation rate, denaturation and structural features of the enzymes under various temperatures. It was found that P. leiognathi luciferase

SUBMITTER: Deeva AA 

PROVIDER: S-EPMC9332260 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature