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Molecular Characterization of UDP-N-Acetylglucosamine Pyrophosphorylase and Its Role in the Growth and Development of the White-Backed Planthopper Sogatella furcifera (Hemiptera: Delphacidae).

ABSTRACT: UDP-N-acetylglucosamine pyrophosphorylase (UAP) is a key enzyme in the chitin biosynthesis pathway of insects. Here, we described the gene SfUAP in the white-backed planthopper Sogatella furcifera (Horváth) with an open reading frame of 1470 bp. Quantitative real-time polymerase chain reaction (qPCR) suggested that SfUAP exhibits a different developmental expression pattern and a higher expression after molting. The highest expression of SfUAP was observed in the integument tissues of adults, whereas head tissues showed negligible expression. RNAi-based gene silencing decreased the mRNA transcript levels in S. furcifera nymphs injected with double-stranded RNA of SfUAP. Finally, SfUAP silencing led to 84% mortality and malformed phenotypes in nymphs. Thus, our results can help better understand the role of SfUAP in S. furcifera.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC9332809 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Molecular Characterization of UDP-<i>N</i>-Acetylglucosamine Pyrophosphorylase and Its Role in the Growth and Development of the White-Backed Planthopper <i>Sogatella furcifera</i> (Hemiptera: Delphacidae).

Wang Zhao Z   Long Gui-Yun GY   Zhou Cao C   Jin Dao-Chao DC   Yang Hong H   Yang Wen-Jia WJ  

Genes 20220727 8

UDP-<i>N</i>-acetylglucosamine pyrophosphorylase (UAP) is a key enzyme in the chitin biosynthesis pathway of insects. Here, we described the gene <i>SfUAP</i> in the white-backed planthopper <i>Sogatella furcifera</i> (Horváth) with an open reading frame of 1470 bp. Quantitative real-time polymerase chain reaction (qPCR) suggested that <i>SfUAP</i> exhibits a different developmental expression pattern and a higher expression after molting. The highest expression of <i>SfUAP</i> was observed in t  ...[more]

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