Decoding the activated stem cell phenotype of the neonatally maturing pituitary.

ABSTRACT: The pituitary represents the endocrine master regulator. In mouse, the gland undergoes active maturation immediately after birth. Here, we in detail portrayed the stem cell compartment of neonatal pituitary. Single-cell RNA-sequencing pictured an active gland, revealing proliferative stem as well as hormonal (progenitor) cell populations. The stem cell pool displayed a hybrid epithelial/mesenchymal phenotype, characteristic of development-involved tissue stem cells. Organoid culturing recapitulated the stem cells' phenotype, interestingly also reproducing their paracrine activity. The pituitary stem cell-activating interleukin-6 advanced organoid growth, although the neonatal stem cell compartment was not visibly affected in Il6^-/- mice, likely due to cytokine family redu

SUBMITTER: Laporte E 

PROVIDER: S-EPMC9333987 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature