Global analysis of cytosine and adenine DNA modifications across the tree of life.

ABSTRACT: Interpreting the function and metabolism of enzymatic DNA modifications requires both position-specific and global quantities. Sequencing-based techniques that deliver the former have become broadly accessible, but analytical methods for the global quantification of DNA modifications have thus far been applied mostly to individual problems. We established a mass spectrometric method for the sensitive and accurate quantification of multiple enzymatic DNA modifications. Then, we isolated DNA from 124 archean, bacterial, fungal, plant, and mammalian species, and several tissues and created a resource of global DNA modification quantities. Our dataset provides insights into the general nature of enzymatic DNA modifications, reveals unique biological cases, and provides complementary quantitati

SUBMITTER: Varma SJ 

PROVIDER: S-EPMC9333990 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature