Ocrelizumab quantitation by liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Introduction

Ocrelizumab is a monoclonal anti-CD20 antibody approved for the treatment of multiple sclerosis (MS). The clinical value of therapeutic drug monitoring (TDM) for this antibody in treatment of MS is unknown, and an adequately specific and precise quantitation method for ocrelizumab in patient serum could facilitate investigation. Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantitation methods have been shown to have higher analytic specificity and precision than enzyme-linked immunosorbent assays.

Objectives

To establish and validate an LC-MS/MS-based quantitation method for ocrelizumab.

Methods

We present an LC-MS/MS-based quantitation method using immunocapture purification followed by trypsinization and analysis by a triple quadrupo

SUBMITTER: Hallin EI 

PROVIDER: S-EPMC9334332 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature