Accurate automatic object 4D tracking in digital in-line holographic microscopy based on computationally rendered dark fields.

ABSTRACT: Building on Gabor seminal principle, digital in-line holographic microscopy provides efficient means for space-time investigations of large volumes of interest. Thus, it has a pivotal impact on particle tracking that is crucial in advancing various branches of science and technology, e.g., microfluidics and biophysical processes examination (cell motility, migration, interplay etc.). Well-established algorithms often rely on heavily regularized inverse problem modelling and encounter limitations in terms of tracking accuracy, hologram signal-to-noise ratio, accessible object volume, particle concentration and computational burden. This work demonstrates the DarkTrack algorithm-a new approach to versatile, fast, precise, and robust 4D holographic tracking based on deterministic computationa

SUBMITTER: Rogalski M 

PROVIDER: S-EPMC9334364 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature