Application of the 3C Method to Study the Developmental Genes in Drosophila Larvae.

ABSTRACT: A transition from one developmental stage to another is accompanied by activation of developmental programs and corresponding gene ensembles. Changes in the spatial conformation of the corresponding loci are associated with this activation and can be investigated with the help of the Chromosome Conformation Capture (3C) methodology. Application of 3C to specific developmental stages is a sophisticated task. Here, we describe the use of the 3C method to study the spatial organization of developmental loci in Drosophila larvae. We critically analyzed the existing protocols and offered our own solutions and the optimized protocol to overcome limitations. To demonstrate the efficiency of our procedure, we studied the spatial organization of the developmental locus Dad in 3rd inst

SUBMITTER: Bylino OV 

PROVIDER: S-EPMC9335292 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature