Superresolution microscopy localizes endogenous Dvl2 to Wnt signaling-responsive biomolecular condensates.

ABSTRACT: During organismal development, homeostasis, and disease, Dishevelled (Dvl) proteins act as key signaling factors in beta-catenin-dependent and beta-catenin-independent Wnt pathways. While their importance for signal transmission has been genetically demonstrated in many organisms, our mechanistic understanding is still limited. Previous studies using overexpressed proteins showed Dvl localization to large, punctate-like cytoplasmic structures that are dependent on its DIX domain. To study Dvl's role in Wnt signaling, we genome engineered an endogenously expressed Dvl2 protein tagged with an mEos3.2 fluorescent protein for superresolution imaging. First, we demonstrate the functionality and specificity of the fusion protein in beta-catenin-dependent and beta-catenin-independent signaling us

SUBMITTER: Schubert A 

PROVIDER: S-EPMC9335300 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature