Biophysical Regulations of Epigenetic State and Notch Signaling in Neural Development Using Microgroove Substrates.

ABSTRACT: A number of studies have recently shown how surface topography can alter the behavior and differentiation patterns of different types of stem cells. Although the exact mechanisms and molecular pathways involved remain unclear, a consistent portion of the literature points to epigenetic changes induced by nuclear remodeling. In this study, we investigate the behavior of clinically relevant neural populations derived from human pluripotent stem cells when cultured on polydimethylsiloxane microgrooves (3 and 10 μm depth grooves) to investigate what mechanisms are responsible for their differentiation capacity and functional behavior. Our results show that microgrooves enhance cell alignment, modify nuclear geometry, and significantly increase cellular stiffness, which we were able to measure

SUBMITTER: Hsu CC 

PROVIDER: S-EPMC9335410 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature