Microbial DNA enrichment promotes liver steatosis and fibrosis in the course of non-alcoholic steatohepatitis.

ABSTRACT:

Aim

Low-grade inflammation is the hallmark of non-alcoholic fatty liver diseases (NAFLD) and non-alcoholic steatohepatitis (NASH). The leakage of microbiota-derived products can contribute to liver inflammation during NAFLD/NASH development. Here, we assessed the roles of gut microbial DNA-containing extracellular vesicles (mEVs) in regulating liver cellular abnormalities in the course of NAFLD/NASH.

Methods

We performed studies with Vsig4^-/- , C3^-/- , cGAS^-/- , and their wild-type littermate mice. Vsig4+ macrophage population and bacterial DNA abundance were examined in both mouse and human liver by either flow cytometric or immunohistochemistry analysis. Gut mEVs were adoptively transferred into Vsig4^-/- , C3^-/- , cGAS

SUBMITTER: Luo Z 

PROVIDER: S-EPMC9335517 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature